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Mouse monoclonal antibody Anti-Human CBP
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| Acetyltransferase enzyme. Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. CREBBP, as coactivator, augments the activity of phosphorylated CREB to activate transcription of cAMP-responsive genes. [UniProtKB¡¡Function] |
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Detection of CBP by Western blot.
Samples: Whole cell lysate from human HeLa (H, 50 μg) and mouse NIH3T3 (M, 50 μg) cells. [Lot No. CBP5I001-1]
Predicted molecular weight: 265 kDa |
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Western blot protocol
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Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CBP mAb. [Lot No. CBP5I001-1]
Predicted molecular weight: 265 kDa |
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Immunoprecipitation protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CBP mAb. [Lot No. CBP5I001-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-CBP monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
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| Accession No. |
U47741 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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