|
Mouse monoclonal antibody Anti-Human GTF2IRD1
|
|
|
|
|
| The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing of this gene generates at least 2 transcript variants. [NCBI Entrez Gene Summary] |
|
|
|
|
|
|
Detection of human GTF2IRD1 by Western blot.
Sample: Whole cell lysate was obtained from HeLa cells transiently transfected with vector (lanes 1, 3) or myc-tagged human GTF2IRD1 (lanes 2, 4). Primary antibodies: anti-myc tag (1 μg/ml) (lanes 1, 2) or ant
Predicted molecular weight: 106 kDa |
| |
Western blot protocol
|
|
 |
|
|
Immunoprecipitation: RIPA lysate of HeLa cells transfected with vector (lanes 1, 3) or myc-tagged GTF2IRD1 (lanes 2, 4) was incubated with anti-GTF2IRD1 (BMR00190) conjugated with protein G sepharose. Immunocomplex was analyzed by Western blot with anti-m
Predicted molecular weight: 106 kDa |
| |
Immunoprecipitation protocol
|
|
|
|
|
|
Localization of human GTF2IRD1 in HeLa cells.
HeLa cells transiently transfected with the myc-tagged GTF2IRD1 expression vector were fixed and stained with anti- GTF2IRD1 (BMR00190) and Hoechst 33342. Human GTF2IRD1 fluorescence signals were observed exc |
| |
Immunocytochemistry protocol
|
|
|
|
|
|
|
|
|
|
Western blot analysis of immunized recombinant protein, using anti-GTF2IRD1 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
|
|
|
| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
GTF3, RBAP2, CREAM1, MUSTRD1, WBSCR11, WBSCR12, hMusTRD1alpha1 |
| Accession No. |
NM_016328 |
| Purchase |
Ordering information
|
|
|
|
|
| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
|
|
|
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
|
|
|
|
close
