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Mouse monoclonal antibody Anti-Human ADAR
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| This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [NCBI Entrez Gene Summary] |
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Detection of human ADAR by Western blot.
Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2010C4a-1]
Predicted molecular weight: 135 kDa |
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Western blot protocol
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Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ADAR mAb. [Lot No. 2010C4a-1]
Predicted molecular weight: 135 kDa |
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Immunoprecipitation protocol
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HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ADAR mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2010C4a-1] |
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Flow cytometry protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ADAR mAb. [Lot No. 2010C4a-1] |
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Immunocytochemistry protocol
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Left : Western blot analysis of immunized recombinant protein, using anti-ADAR monoclonal antibody.
Right : CBB staining of immunized recombinant protein.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
DSH, G1P1, IFI4, p136, ADAR1, DRADA,DSRAD, IFI-4, K88dsRBP |
| Accession No. |
NM_001111 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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