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Mouse monoclonal antibody Anti-Human DDX27
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| DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [NCBI Entrez Gene Summary] |
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Detection of DDX27 by Western blot.
Samples: Whole cell lysate from human HeLa (H, 25 μg) and mouse NIH3T3 (M, 25 μg) cells. [Lot No. 2251C2a-1]
Predicted molecular weight: 89 kDa |
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Western blot protocol
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HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DDX27 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2251C2a-1] |
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Flow cytometry protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX27 mAb. [Lot No. 2251C2a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-DDX27 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
RHLP, Rrp3p, PP3241, HSPC259, MGC1018, FLJ12917,FLJ20596, FLJ22238, dJ686N3.1, DKFZp667N057 |
| Accession No. |
NM_017895 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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