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Mouse monoclonal antibody Anti-Human DDB2
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| Plays a role in DNA repair by forming with DDB1 the UV-damaged DNA-binding protein complex (UV-DDB). Binds to pyrimidine dimers. Component of the RBX1-CUL4-DDB2 ubiquitin ligase. Required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. [UniProtKB¡¡Function] |
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Detection of human DDB2 by Western blot.
Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2246C4a-1]
Predicted molecular weight: 47 kDa |
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Western blot protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDB2 mAb. [Lot No. 2246C4a-1] |
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Immunocytochemistry protocol
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Left : Western blot analysis of immunized recombinant protein, using anti-DDB2 monoclonal antibody.
Right : CBB staining of immunized recombinant protein.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
FLJ34321 |
| Accession No. |
NM_000107 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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