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Mouse monoclonal antibody Anti-Human NCOA2

Catalog No. BMR00522 ¡Î2615C1a¡Ï Data Sheet  

¢£Background 
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA2 to control energy balance between white and brown adipose tissues. [NCBI Entrez Gene Summary]

¢£Application Data
Detection of NCOA2 by Western blot. Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2615C1a-1]

Predicted molecular weight: 159 kDa
  Western blot protocol 

¢£Quality of Control
Western blot analysis of immunized recombinant protein, using anti-NCOA2 monoclonal antibody.


Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Antibody Concentration 100 μg/ml (1.0 ml)
Host Species Human
Preparation This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility Filtered through a 0.22 μm membrane.
Alternate Names TIF2,GRIP1, NCoA-2, MGC138808
Accession No. NM_006540
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Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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