|
Mouse monoclonal antibody Anti-Human RELA
|
|
|
|
|
| NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] [NCBI Entrez Gene Summary] |
|
|
|
|
|
|
Detection of RELA by Western blot.
Samples: Whole cell lysate from human HeLa (H, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 340C1a-1]
Predicted molecular weight: 60 kDa |
| |
Western blot protocol
|
|
 |
|
|
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RELA mAb. [Lot No. 340C1a-1] |
| |
Immunocytochemistry protocol
|
|
|
|
|
|
|
|
|
|
Western blot analysis of immunized recombinant protein, using anti-RELA monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
|
|
|
| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
|
| Accession No. |
BC014095 |
| Purchase |
Ordering information
|
|
|
|
|
| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
|
|
|
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
|
|
|
|
close
