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Mouse monoclonal antibody Anti-Human NEIL2

Catalog No. BMR00604 ¡Î2626C2a¡Ï Data Sheet  

¢£Background 
NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).[supplied by OMIM] [NCBI Entrez Gene Summary]

¢£Application Data
Detection of NEIL2 by Western blot. Samples: Whole cell lysate from human HeLa (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2626C2a-1]

Predicted molecular weight: 36 kDa
  Western blot protocol 

¢£Quality of Control
Western blot analysis of immunized recombinant protein, using anti-NEIL2 monoclonal antibody.


Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.
Antibody Concentration 100 μg/ml (1.0 ml)
Host Species Human
Preparation This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility Filtered through a 0.22 μm membrane.
Alternate Names NEH2, MGC2832, MGC4505, FLJ31644
Accession No. NM_145043
Purchase Ordering information
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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