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Mouse monoclonal antibody Anti-Human HD
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| Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative
disorder characterized by loss of striatal neurons. This is thought to be caused by
an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates
as a polyglutamine repeat in the protein product. A fairly broad range in the
number of trinucleotide repeats has been identified in normal controls, and repeat
numbers in excess of 40 have been described as pathological. The huntingtin locus
is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely
expressed and is required for normal development. It is expressed as 2
alternatively polyadenylated forms displaying different relative abundance in
various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is
expressed predominantly in adult and fetal brain whereas the smaller transcript of
approximately 10.3 kb is more widely expressed. The genetic defect leading to
Huntington's disease may not necessarily eliminate transcription, but may confer a
new property on the mRNA or alter the function of the protein. One candidate is the
huntingtin-associated protein-1, highly expressed in brain, which has increased
affinity for huntingtin protein with expanded polyglutamine repeats. This gene
contains an upstream open reading frame in the 5' UTR that inhibits expression of
the huntingtin gene product through translational repression.[NCBI Entrez Gene Summary] |
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HD mAb. [Lot No. 2401C1a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-HD monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
HTT, IT15 |
| Accession No. |
NM_002111 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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