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Mouse monoclonal antibody Anti-Human HDAC1
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| Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a
key role in the regulation of eukaryotic gene expression. The protein encoded by
this gene belongs to the histone deacetylase/acuc/apha family and is a component
of the histone deacetylase complex. It also interacts with retinoblastoma tumorsuppressor
protein and this complex is a key element in the control of cell
proliferation and differentiation. Together with metastasis-associated protein-2, it
deacetylates p53 and modulates its effect on cell growth and apoptosis.[NCBI Entrez Gene Summary] |
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Detection of HDAC1 by Western blot.
Samples: Whole cell lysate from human HeLa (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 730C6a-1]
Predicted molecular weight: 55 kDa |
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Western blot protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-HDAC1 mAb. [Lot No.730C6a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-HDAC1 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203 |
| Accession No. |
NM_004964 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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