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Mouse monoclonal antibody Anti-Human APEX1
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| Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous
hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific
abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA
replication so the cell contains systems to identify and repair such sites. Class II AP
endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene
encodes the major AP endonuclease in human cells. Splice variants have been
found for this gene; all encode the same protein.[NCBI Entrez Gene Summary] |
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Detection of APEX1B by Western blot.
Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2027C2a-1]
Predicted molecular weight: 36 kDa |
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Western blot protocol
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Western blot analysis of immunized recombinant protein, using anti-APEX1 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
APE, APX, APE1, APEN, APEX, HAP1, REF1, APE-1, REF-1 |
| Accession No. |
NM_001641 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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