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Mouse monoclonal antibody Anti-Human CCNA2
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| The protein encoded by this gene belongs to the highly conserved cyclin family,
whose members are characterized by a dramatic periodicity in protein abundance
through the cell cycle. Cyclins function as regulators of CDK kinases. Different
cyclins exhibit distinct expression and degradation patterns which contribute to the
temporal coordination of each mitotic event. In contrast to cyclin A1, which is
present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin
binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S
and G2/M transitions.[NCBI Entrez Gene Summary] |
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Detection of CCNA2 by Western blot.
Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2112C2a-1]
Predicted molecular weight: 49 kDa |
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Western blot protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CCNA2 mAb. [Lot No.2112C2a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-CCNA2 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
CCN1, CCNA |
| Accession No. |
NM_001237 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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