|
Mouse monoclonal antibody Anti-Human CCNE1
|
|
|
|
|
| The protein encoded by this gene belongs to the highly conserved cyclin family,
whose members are characterized by a dramatic periodicity in protein abundance
through the cell cycle. Cyclins function as regulators of CDK kinases. Different
cyclins exhibit distinct expression and degradation patterns which contribute to the
temporal coordination of each mitotic event. This cyclin forms a complex with and
functions as a regulatory subunit of CDK2, whose activity is required for cell cycle
G1/S transition. This protein accumulates at the G1-S phase boundary and is
degraded as cells progress through S phase. Overexpression of this gene has been
observed in many tumors, which results in chromosome instability, and thus may
contribute to tumorigenesis. This protein was found to associate with, and be
involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the
ATM locus), which participates in cell-cycle regulated histone gene expression and
plays a critical role in promoting cell-cycle progression in the absence of pRB. Two
alternatively spliced transcript variants of this gene, which encode distinct isoforms,
have been described. Two additional splice variants were reported but detailed
nucleotide sequence information is not yet available.[NCBI Entrez Gene Summary] |
|
|
|
|
|
|
Detection of human CCNE1 by Western blot.
Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2121C1a-1]
Predicted molecular weight: 47 kDa |
| |
Western blot protocol
|
|
 |
|
|
|
|
|
|
Western blot analysis of immunized recombinant protein, using anti-CCNE1 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
|
|
|
| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
CCNE |
| Accession No. |
NM_001238 |
| Purchase |
Ordering information
|
|
|
|
|
| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
|
|
|
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
|
|
|
|
close
