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Mouse monoclonal antibody Anti-Human MCM2
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| The protein encoded by this gene is one of the highly conserved mini-chromosome
maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome
replication. The hexameric protein complex formed by MCM proteins is a key
component of the pre-replication complex (pre_RC) and may be involved in the
formation of replication forks and in the recruitment of other DNA replication related
proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to
regulate the helicase activity of the complex. This protein is phosphorylated, and
thus regulated by, protein kinases CDC2 and CDC7.[NCBI Entrez Gene Summary] |
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Detection of human MCM2 by Western blot.
Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2546C1a-1]
Predicted molecular weight: 102 kDa |
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Western blot protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MCM2 mAb. [Lot No.2546C1a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-MCM2 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
BM28, CCNL1, CDCL1, cdc19, D3S3194, MITOTIN, KIAA0030, MGC10606 |
| Accession No. |
NM_004526 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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