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Mouse monoclonal antibody Anti-Human RAD23B
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| The protein encoded by this gene is one of two human homologs of Saccharomyces
cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This
protein was found to be a component of the protein complex that specifically
complements the NER defect of xeroderma pigmentosum group C (XP-c) cell
extracts in vitro. This protein was also shown to interact with, and elevate the
nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which
suggested a role in DNA damage recognition in base excision repair. This protein
contains an N-terminal ubiquitin-like domain, which was reported to interact with
26S proteasome, and thus this protein may be involved in the ubiquitin mediated
proteolytic pathway in cells.[NCBI Entrez Gene Summary] |
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Detection of human RAD23B by Western blot.
Samples: Whole cell lysate (25 μg) from HeLa cells. [Lot No. 2857D7a-1]
Predicted molecular weight: 43 kDa |
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Western blot protocol
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Western blot analysis of immunized recombinant protein, using anti-RAD23B monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
P58, HR23B, HHR23B |
| Accession No. |
NM_002874 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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