|
Mouse monoclonal antibody Anti-Human BCL3
|
|
|
|
|
| This gene is a proto-oncogene candidate. It is identified by its translocation into the
immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by
this gene contains seven ankyrin repeats, which are most closely related to those found
in I kappa B proteins. This protein functions as a transcriptional co-activator that
activates through its association with NF-kappa B homodimers. The expression of this
gene can be induced by NF-kappa B, which forms a part of the autoregulatory loop that
controls the nuclear residence of p50 NF-kappa B. [provided by RefSeq][NCBI Entrez Gene Summary] |
|
|
|
|
|
|
Detection of BCL3 by Western blot.
Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2069E2a-1 ]
Predicted molecular weight: 47 kDa |
| |
Western blot protocol
|
|
 |
|
|
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BCL3 mAb. [Lot No.2069E2a-1] |
| |
Immunocytochemistry protocol
|
|
|
|
|
|
|
|
|
|
Left : Western blot analysis of immunized recombinant protein, using anti-BCL3 monoclonal antibody.
Right : CBB staining of immunized recombinant protein.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
|
|
|
| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
BCL4, D19S37 |
| Accession No. |
NM_005178 |
| Purchase |
Ordering information
|
|
|
|
|
| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
|
|
|
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
|
|
|
|
|
close
