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Mouse monoclonal antibody Anti-Human DDX17
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| DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are
putative RNA helicases. They are implicated in a number of cellular processes involving
alteration of RNA secondary structure, such as translation initiation, nuclear and
mitochondrial splicing, and ribosome and splicesosome assembly. Based on their
distribution patterns, some members of this family are believed to be involved in
embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a
DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by
dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each
other than to any other member of the DEAD box family. Several alternatively spliced
transcripts encoding different isoforms, some of which use non-AUG (CUG) translation
initiation codon, have been described for this gene. [provided by RefSeq][NCBI Entrez Gene Summary] |
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Detection of DDX17 by Western blot.
Samples: Whole cell lysate from human HEK293 (H, 25 μg) , mouse NIH3T3 (M, 25 μg) and rat F2408 (R, 25 μg) cells. [Lot No. 2248C2a-1]
Predicted molecular weight: 80 kDa |
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Western blot protocol
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Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDX17 mAb. [Lot No.2248C2a-1] |
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Immunocytochemistry protocol
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Western blot analysis of immunized recombinant protein, using anti-DDX17 monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
P72, RH70, DKFZp761H2016 |
| Accession No. |
NM_006386 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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