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Mouse monoclonal antibody Anti-Human DFFA
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| Apoptosis is a cell death process that removes toxic and/or useless cells during
mammalian development. The apoptotic process is accompanied by shrinkage and
fragmentation of the cells and nuclei and degradation of the chromosomal DNA into
nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD
(DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers
DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by
caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component
of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin
condensation during apoptosis. Two alternatively spliced transcript variants encoding
distinct isoforms have been found for this gene. [provided by RefSeq][NCBI Entrez Gene Summary] |
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Western blot analysis of immunized recombinant protein, using anti-DFFA monoclonal antibody.
Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids. |
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| Antibody Concentration |
100 μg/ml (1.0 ml) |
| Host Species |
Human |
| Preparation |
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. |
| Sterility |
Filtered through a 0.22 μm membrane. |
| Alternate Names |
DFF1, ICAD, DFF-45 |
| Accession No. |
NM_213566 |
| Purchase |
Ordering information
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| Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off . |
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FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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